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Obtaining Serum from frozen EDTA blood or cleaning reddish serum?

Serum Frozen Blood EDTA Hemolysis

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#1 hercolanium

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Posted 14 June 2014 - 02:18 AM

Hi, I have two tubes from the same patient; one is gel-serum tube (yellow cap), the other is EDTA whole blood (mauve cap), when I centrifuged the gel-serum tube the serum came out bloody (reddish). I need the serum for Vitamin analysis, can I obtain another serum from the frozen whole blood tube or is there is a way to clean the serum sample?

 

Please help.

 

P.S. The color of the resulting serum is between the two colors in the image attached

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  • Hemolysis_tubes_Edit.jpg


#2 mdfenko

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Posted 16 June 2014 - 04:26 AM

how are you performing your vitamin analysis?

 

when i did it, i precipitated the proteins (it was a long time ago so i'm not sure, but i think i used acetonitrile to precipitate). that eliminated the hemoglobin from the serum.


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