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washing pellets; recovery of isolates in glycerol


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#1 Dima

Dima

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Posted 13 June 2014 - 11:07 AM

Hello ! Could you pls help me know more on this?

1- after 24 hrs incubation of colonies in a broth, the culture is centrifuged, supernatant discarded, pellets washed and resuspendedbin broth. What is the purpose of centrifugion, washing? To get rid of dead cells?? Or nutrients outproducts?

2- i have e.coli isolates in glycerol.what s the best way to recover? I have rapid e.coli agar from biorad. Shall i take a loopful from the surface and suspend in brain heart infusion broth? And later Shall i streak the colonies on the agar or follow pour plate techniques??

Thanks loads in advance for the help!




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