Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

ABC (Avidin Biotin Complex) in ELISA


  • Please log in to reply
1 reply to this topic

#1 bdg25

bdg25

    member

  • Active Members
  • Pip
  • 9 posts
0
Neutral

Posted 11 June 2014 - 07:50 AM

Hi All,

has anyone had any success using ABC (avidin-biotin complex) to improve the sensitivity of their ELISA? I am trying the Vector labs  VECTASTAIN Elite ABC Kit and I'm getting significantly worse results than just using standard biotin followed by strep-HRP. Does anyone have any successful experience with this? There are publications reporting its efficacy but not much in the ways of specific protocols, and using the one that came with the product is not working for me. Thanks

Ben



#2 mdfenko

mdfenko

    an elder

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 2,704 posts
123
Excellent

Posted 12 June 2014 - 04:07 AM

i used one a long time ago. i followed the instructions as printed and was rewarded with good results.


talent does what it can
genius does what it must
i do what i get paid to do




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.