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Biotin Conjugation (Biotinylation) Kit Recommendations

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#1 bdg25



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Posted 11 June 2014 - 07:46 AM

Hi All,

do you have any particular biotinylation kits that you recommend?  I have been using the pierce biotinylation but my biotinylated antibody followed by streptavidin HRP is performing worse than my directly HRP conjugated detection antibody. I would expect them to be equal at worst, but I'm losing about 1 order or magnitude of sensitivity so I'm skeptical of my biotinylation. Any suggestions?



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