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centrifuge parameter to obtain bacteria in pellet


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#1 paa6

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Posted 10 June 2014 - 11:35 PM

hello friends, 

I am a complete newbie in wet lab. I want to know that what centrifuge parameters should I give to get bacteria in pellets. I don't want to lyse bacteria. I just want to extract MRS medium and discard bacteria. I was thinking to try 6000-7000 rpm for 5 min. 

 



#2 El Crazy Xabi

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Posted 10 June 2014 - 11:55 PM

Depends on the sedimentation speed of the bacteria, how many bacteria would you like to leave on the supernatant,... in any case, bacteria aren't that fragile but would depend on the species too

 

 

 

PS: if you can use xg or rcf, way better than rpm, that's only useful for a given centrifuge and rotor.






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