Note sure if this is the right sub-forum for this question - in case it is, kindly let me know and I'll move it. I need some help with understanding genotype of a strain that we're using for protein expression
I'm currently using a Keio collection strain from CGSC (Strain BW25113, CGSC# 7636).
Genotype is as follows:
- Sex(Hfr,F+,F-,or F'): F-
- 6 Mutations:
I was wondering if this strain could be used for arabinose induced expression. The authors/creators of the Keio collection seemed to have used L-arabinose to induce expression of a vector transformed into this strain.
A more fundamental question would be : will the lack of genes starting 25bp upstream of araB start codon all the way up to 8bp following araD start codon, while leaving the araBAD promoter intact and the araC gene intact allow this strain to be used with a pBAD vector for a tightly regulated expression system?
Edited by ags2014, 10 June 2014 - 09:48 AM.