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Alternatives to mouse antibody for mouse tissue


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#1 blixx

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Posted 10 June 2014 - 03:57 AM

Hi all

 

I have mouse tissues, extracted protein and would like to run a western blot to detect my protein of interest. However, the only good antibody for this protein is anti-mouse (works really well on human cell lines). The rabbit version is really bad, loads of unspecific binding and should not be used at all. Is there a way around using anti-mouse antibody on mouse tissues?



#2 mdfenko

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Posted 10 June 2014 - 04:17 AM

do you mean that the antibody is produced in mouse ("anti-mouse" would be exactly what you need)?

 

is there any way that you could adjust conditions (eg dilution, tween concentration, salt concentration, pH, preabsorption) to use the rabbit antibody effectively?


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