Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

Alternatives to mouse antibody for mouse tissue

  • Please log in to reply
1 reply to this topic

#1 blixx



  • Active Members
  • Pip
  • 8 posts

Posted 10 June 2014 - 03:57 AM

Hi all


I have mouse tissues, extracted protein and would like to run a western blot to detect my protein of interest. However, the only good antibody for this protein is anti-mouse (works really well on human cell lines). The rabbit version is really bad, loads of unspecific binding and should not be used at all. Is there a way around using anti-mouse antibody on mouse tissues?

#2 mdfenko


    an elder emeritus

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 3,259 posts

Posted 10 June 2014 - 04:17 AM

do you mean that the antibody is produced in mouse ("anti-mouse" would be exactly what you need)?


is there any way that you could adjust conditions (eg dilution, tween concentration, salt concentration, pH, preabsorption) to use the rabbit antibody effectively?

talent does what it can
genius does what it must
i do what i get paid to do

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.