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Blocking agent in western blotting of histone proteins

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#1 biochemscientist



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Posted 09 June 2014 - 03:53 AM

Dear Friends,

I am doing western blotting of histone proteins and their post translational modifications using ABCAM antibodies. I wanted to ask what should i use as a blocking agent. Normally i have used 5% BSA as a blocking agent in my other blotting experiments other than histones


Regards :)

#2 bob1


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Posted 09 June 2014 - 03:44 PM

According to the Odyssey people, BSA should be avoided in most cases as it tends to give more non-specific binding.  Try 3-5% non-fat milk powder in PBS-T or TBS-T.

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