Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Blocking agent in western blotting of histone proteins


  • Please log in to reply
1 reply to this topic

#1 biochemscientist

biochemscientist

    member

  • Active Members
  • Pip
  • 23 posts
1
Neutral

Posted 09 June 2014 - 03:53 AM

Dear Friends,

I am doing western blotting of histone proteins and their post translational modifications using ABCAM antibodies. I wanted to ask what should i use as a blocking agent. Normally i have used 5% BSA as a blocking agent in my other blotting experiments other than histones

 

Regards :)



#2 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 5,835 posts
415
Excellent

Posted 09 June 2014 - 03:44 PM

According to the Odyssey people, BSA should be avoided in most cases as it tends to give more non-specific binding.  Try 3-5% non-fat milk powder in PBS-T or TBS-T.






Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.