Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

expired medium

medium

  • Please log in to reply
5 replies to this topic

#1 medibio

medibio

    member

  • Active Members
  • Pip
  • 25 posts
0
Neutral

Posted 04 June 2014 - 11:40 PM

Hi,

 

I have some expired medium (RPMI 1640 and DMEM). In your opinion, can I use them? 



#2 science noob

science noob

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 281 posts
20
Excellent

Posted 05 June 2014 - 04:46 AM

Depending on how long 'expired' they are. I would recommend no, but if your lab in low on funds, maybe. But for important experiments I would say, go the safer side and get a new fresh bottle. These media have growth supplements that could break down with time.



#3 medibio

medibio

    member

  • Active Members
  • Pip
  • 25 posts
0
Neutral

Posted 05 June 2014 - 12:04 PM

Expired rougly 1 year.

 

What about tripsin EDTA solution? Its expired time is rougly 3 months.



#4 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 5,831 posts
415
Excellent

Posted 05 June 2014 - 01:05 PM

Trypsin will probably be fine, the enzyme is quite stable.



#5 science noob

science noob

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 281 posts
20
Excellent

Posted 06 June 2014 - 03:11 AM

Expired rougly 1 year.

 

What about tripsin EDTA solution? Its expired time is rougly 3 months.

 

I would think twice if it's 1 year overdue. 



#6 medibio

medibio

    member

  • Active Members
  • Pip
  • 25 posts
0
Neutral

Posted 06 June 2014 - 06:50 AM

I will dare to use Tripsin EDTA but not medium







Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.