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Effective inhibitor of cell proliferation


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4 replies to this topic

#1 science noob

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Posted 04 June 2014 - 12:49 AM

Does anyone know or worked with inhibitors which I can supplement into my cell culture to stop cell proliferation? 

It is also important that the inhibitor will not effect cell viability to keep the cells alive so I can run experiments on them.

 

 



#2 bob1

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Posted 04 June 2014 - 02:07 AM

Try a double thymidine block.



#3 gfischer

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Posted 04 June 2014 - 07:31 AM

You could also try Cytosine beta-D-arabinofuranoside or 5-Fluoro-2'-deoxyuridine.  Both block DNA replication, by different mechanisms.

 

In theory they shouldn't affect viability or cellular function, but you'll have to do some titration to find an acceptable concentration.


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#4 science noob

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Posted 04 June 2014 - 03:58 PM

Thanks for the replies.  One other important thing I have to ask is how permanent is the inhibition? Few hours/days/permanent?



#5 bob1

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Posted 04 June 2014 - 07:48 PM

Double thymidine will block for as long as you keep it on I think.






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