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thionin extraction

thionin protein extraction

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3 replies to this topic

#1 ad404

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Posted 03 June 2014 - 10:25 PM

hello everyone

 

can anybody tell me about thionin extraction from fungal infection induced barley seeds? mol wt 4.5 to 5 kda. Is it possible to give ammonium sulfate cuts?? more importantly, after homogenization, how is it possible to concentrate the protein using methods other than ammonim sulfate cuts ie salting out?


Edited by ad404, 03 June 2014 - 10:25 PM.


#2 mdfenko

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Posted 04 June 2014 - 04:10 AM

to concentrate without salt (eg ammonium sulfate) you can use an ultrafilter (like this one from millipore, with a 1kDa cutoff).


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#3 ad404

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Posted 06 June 2014 - 01:31 AM

thanks mdfenko

 

yes ultrafiltration is a good approach. but I was looking for cheaper methods of concentrating protein from cell lysates. do you think using a spin column is a good idea?



#4 mdfenko

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Posted 06 June 2014 - 04:23 AM

if you mean spin "filtration" then yes. the only possible problem is that the lowest cutoff i can find (at millipore) is 3 kDa. that may be a problem for your protein.

 

here's the page at millipore for 3 kDa cutoff spin filters.


talent does what it can
genius does what it must
i do what i get paid to do





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