Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

KIR gene promoters for MSP primers design

promoter msp methylation

  • Please log in to reply
No replies to this topic

#1 Lj Lee

Lj Lee

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 03 June 2014 - 05:23 AM

Hi all, 

 

Good day, I'm new here and trying to create a simple MSP for my few of my target genes which are KIR (Killer cell immunoglobulin-like receptor) located in chromosome 19. I know most of the researcher using MethPrimer software to design the M and U primers but I met some difficulties to design the primers as there were no CpG island found in the 'promoter sequence'. 

 

I get the 'promoter' of KIR2DL2 from Ensembl : http://www.ensembl.o...09.1:7891-96246 (the sequence before first exon that highlighted in pink) however when I copied and pasted it to MethPrimer, it showed no CpG island found in the input sequence.

 

Does anyone know how to identify the promoter sequence of a particular gene? I'm totally lost and stuck at this stage.

 

Thanks. smile.png


Edited by Lj Lee, 03 June 2014 - 05:24 AM.






Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.