Good day, I'm new here and trying to create a simple MSP for my few of my target genes which are KIR (Killer cell immunoglobulin-like receptor) located in chromosome 19. I know most of the researcher using MethPrimer software to design the M and U primers but I met some difficulties to design the primers as there were no CpG island found in the 'promoter sequence'.
I get the 'promoter' of KIR2DL2 from Ensembl : http://www.ensembl.o...09.1:7891-96246 (the sequence before first exon that highlighted in pink) however when I copied and pasted it to MethPrimer, it showed no CpG island found in the input sequence.
Does anyone know how to identify the promoter sequence of a particular gene? I'm totally lost and stuck at this stage.
Edited by Lj Lee, 03 June 2014 - 05:24 AM.