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Decreasing efficiency with higher probe concentration

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#1 kaja.bieganska



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Posted 03 June 2014 - 02:16 AM

I've got a problem with my q-pcr. When I run a standard curve with 250 nm probe the reaction efficiency is rather poor. Decreasing the probe amount on the other hand results in amplification in my NTC. Do you have any suggestions how to optimize this reaction? Thanks a lot.




please only one post per problem/topic, thanks.


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