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Preservation of bacteria in PBS for immunization


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#1 ronny1

ronny1

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Posted 24 June 2004 - 01:32 AM

I want to grow the culture of recombinant Lactococcus lactis for the purpose of immunization. After centrifugation and resuspension of the bacteria to a particular concentration in PBS (pH 7.2), can I preserve it somehow (say at 5C or -80C) for reuse as booster later or should I grow fresh bacteria each time for booster?




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