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Biotin-streptavidin signal amplification

biotin streptavidin

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#1 Ears



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Posted 02 June 2014 - 09:49 AM



I'm using a biotinylated primary antibody, followed by PE-conjugated streptavidin. I've heard that using streptavidin and biotin can help to amplify signals, but I am right that this is only the case when the biotin is attached to the label? This seems to make sense as one streptavidin can bind four biotin molecules, but I've read conflicting information so I'm not too sure...



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