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BSA vs HSA as serum free culture supplement


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#1 sara.r

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Posted 02 June 2014 - 02:34 AM

Hi

 

I am going to use serum free medium for mammalian cell culture. I have read in literature that recombinant human serum albumin can be used as a supplement and helps cells to grow. But much more expensive than recombinant  BSA. So I was wondering if any one has tried BSA vs HSA in serum free culture.

 

Regards



#2 science noob

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Posted 02 June 2014 - 03:48 AM

Depending on what cell type you're culturing.  BSA is a good serum replacement but some cells need other supplements for optimal growth.  BSA isn't cheap either and making it up can be a hassle. Some companies have come up with serum knockout supplements which come in liquid form that you can add to your medium. 






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