would be great to receive your supportive comments as always ! I have isolated Listeria monocytoegenes and E.coli and I would like to identify the strains using RT-PCR.
Is there any protocol that you could share with me ?? I am reading research papers to understand which primers to use , but is there a special kit that is used to identify the L.Mono and E.Coli?
Any help in this field and any advice on the steps i should take are greatly appreciated!
Moved to microbiology