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orthotopic mouse model of U251 cell line


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#1 liu2046

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Posted 30 May 2014 - 02:54 AM

Hi, everyone.

I'm fresh in developing orthotopic mouse model on the brain.

So I practiced to develop u87/nude mouse glioma model for the preparation of my  research project.

I practiced to develop brain tumor model on three nude mouse. I found no abnormal information 7 days after the surgery. However 2 of them were found dead on the 10th day and now I am so confused.

I just wonder why  two nude mouse died at such a strange time point. 

And i hope to get some advice from experienced person.



#2 Tabaluga

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Posted 30 May 2014 - 05:12 AM

any signs of infection perhaps ? first thing that comes to mind, but don't have any mouse experience.....


Il dort. Quoique le sort fût pour lui bien étrange,
Il vivait. Il mourut quand il n'eut plus son ange;
La chose simplement d'elle-même arriva,
Comme la nuit se fait lorsque le jour s'en va.

 


#3 liu2046

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Posted 02 June 2014 - 04:46 AM

 

any signs of infection perhaps ? first thing that comes to mind, but don't have any mouse experience.....

  I didn't detect apparent abnormality and have no experience either.

#4 Fungus_Dreams

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Posted 30 June 2014 - 05:05 AM

Dehydration quite likley if there is no obvious sign of anything else. With my post-op rodents I like to soak some mouse chow in a bowl of water and then place a small cup of this mushy food into the recovery cage. Some companies also make a pre-formulated "recovery gel" which our lab uses frequently as well.



#5 Fungus_Dreams

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Posted 30 June 2014 - 05:07 AM

And don't forget to give a little nesting material so they can build a shelter/thermoregulate/recover better. Especially important for nude mice :) 






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