I'm fresh in developing orthotopic mouse model on the brain.
So I practiced to develop u87/nude mouse glioma model for the preparation of my research project.
I practiced to develop brain tumor model on three nude mouse. I found no abnormal information 7 days after the surgery. However 2 of them were found dead on the 10th day and now I am so confused.
I just wonder why two nude mouse died at such a strange time point.
And i hope to get some advice from experienced person.