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Any online tool to check the propability of primer dimer formetion _


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4 replies to this topic

#1 Mohamed 1984

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Posted 27 May 2014 - 12:35 PM

Hi,

I am doubt about primer dimer formation in my PCR product. Is there is any online tool to check if my primer might form dimers ? 



#2 bob1

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Excellent

Posted 27 May 2014 - 12:43 PM

Primer3 and PrimerBLAST both do this.



#3 phage434

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Excellent

Posted 27 May 2014 - 06:15 PM

The tool menu at the IDTDNA site has good tools for primer homodimers and heterodimers.
They don't distinguish ones that have 5' overhangs, which have severe consequences, while the 3' overhangs matter little. This is because 5' overhangs can be extended, which produces an incorrect primer, while the 3' overhang cannot be extended.



#4 Mohamed 1984

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Posted 28 May 2014 - 07:33 AM

Thanks



#5 Mohamed 1984

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Posted 28 May 2014 - 05:52 PM

Actually I Already have the primer and I wanted to see the propablity of dimer formation> Is this is helpful 






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