The aim of my experiment is to show the anti-bacterial efficacy of my compound. The study is based on proving antibacterial activity based on a cutaneous infection model. Several papers i have read, report that they are able to quantify bacteria (CFU) from 12-14 day abscess by homogenizing the abscess in selected broths. I have tried to quantify 14 day abscesses but without homogenizing them as I do not have a homogenizer. I had used a syringe and vortexed the tissue as best as I could. I did not get any bacteria growing on my plate.
My question is, aren't abscesses (especially mature ones) made up of dead cells and therefore non-quantifiable?
Or is there something I'm doing wrong?
Please kindly advise.