I would like to isolate tumour cells from the bone marrow in order to run them on a flow cytometer. I have attempted to flush the bone marrow from the tibia of a mouse, and then scraped the bone after cutting it in half.
I have been unable to detect the population of cells I am interested in. I am assuming that the tumour cells are localised to a niche within bone and thus I would need to apply a different approach to extracting these cells. Perhaps through the use of collagenase?
Would anyone happen to have any experience with this, or perhaps shed some light on how to best approach this?