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secretome and zymograms


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#1 _veronica_

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Posted 26 May 2014 - 06:51 AM

Hi everyone

 

I have a question about the study of secreted enzymes by filamentous fungi: as I have to compare kinetic secretome levels , I should make zymograms. Before them I need a protein gel: which one is better: SDS-Page or a NON denaturat.protein gel?  Do you know any protocol about cellulases? thanks a lot , Veronica



#2 mdfenko

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Posted 27 May 2014 - 04:21 AM

non-denaturing is probably better (depends on what you're trying to show). sds-page will require a renaturing step which may not return full activity.


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