Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

Looking for in-house, cheap, reliable, in vitro LPS detection assay

LPS detection

  • Please log in to reply
No replies to this topic

#1 IPI



  • Active Members
  • Pip
  • 13 posts

Posted 24 May 2014 - 09:43 PM

Hi there,


I have prepared a recombinant protein in E. coli and it is extremely important for me to know whether the purified protein contains LPS in order to avoid false-positive interpretations. 


I was wondering: is there any cheap in-house technique (or an affordable kit!) to test the presence of LPS in vitro?


I have to mention I cannot use "The Endpoint Chromogenic Limulus Amebocyte Lysate (LAL) Test" due to the inherent Coomassie Blue color of my recombinant protein.


With many thanks for your responses in advance.

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.