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Looking for in-house, cheap, reliable, in vitro LPS detection assay

LPS detection

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#1 IPI

IPI

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Posted 24 May 2014 - 09:43 PM

Hi there,

 

I have prepared a recombinant protein in E. coli and it is extremely important for me to know whether the purified protein contains LPS in order to avoid false-positive interpretations. 

 

I was wondering: is there any cheap in-house technique (or an affordable kit!) to test the presence of LPS in vitro?

 

I have to mention I cannot use "The Endpoint Chromogenic Limulus Amebocyte Lysate (LAL) Test" due to the inherent Coomassie Blue color of my recombinant protein.

 

With many thanks for your responses in advance.






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