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Miltenyi Monocyte Isolation Kit II

Monocytes negative selection post cryofreezing PBMCs

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#1 PhD KCL

PhD KCL

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Posted 23 May 2014 - 02:20 AM

Hi everyone,

 

I am using the Miltenyi Monocyte negative Isolation kit II. It works beautifully on freshly isolated PBMCs, but when I try to use it on PBMCs after they have been cryofrozen my purity drops to 50-60%. Has anyone had a similar problem and does anyone have any suggestions about how I could increase the purity when using post cryofrozen PBMCs? 

 

Thanks.

 

Laura







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