I want to grow and differentiate human cortical neurons. I have a vial of HCN-2 cells from ATCC. From what I understand, they are extremely slow and difficult to differentiate. Changing my cell type is not a viable choice right now. So I make do and hav a couple of questions. If anybody has any experience either differentiating these or other cortical neurons, I would really appreciate some help.
* The nerve growth factor required to differentiate these cells do they have to be human or mouse would be ok as well? ( apologise if this is a really dumb question!)
* ATCC is no help answering anything as they were stored there and ATCC never differentiated them.
* Do you add the cAMP, NGF and IBMX required for differentiation in the media or fresh to the cells every time?
* is there any coating of Matrigel or collagen I required to help the cells grow better?
Please any and all help would be great. Thanks.