2 replies to this topic

[vesko_baev]

I have the sequence of one RNA (20nt) in plant organism, which strategy is the best for cloning it?

[wliu]

Use to 3' RACE and 5' RACE kits from Invitrogen

[mich_ard]

synthesize 2 primers in complementry,and anneal to each other, then clone. if you like, you can add restriction sites to the primer so that it's easier to put in a vector.

Mike

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