I have the sequence of one RNA (20nt) in plant organism, which strategy is the best for cloning it?
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#2
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Posted 23 June 2004 - 05:09 AM
Use to 3' RACE and 5' RACE kits from Invitrogen
#3
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Posted 23 June 2004 - 06:13 AM
synthesize 2 primers in complementry,and anneal to each other, then clone. if you like, you can add restriction sites to the primer so that it's easier to put in a vector.
Mike
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Mike
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