2 replies to this topic
Posted 23 June 2004 - 05:03 AM
I have the sequence of one RNA (20nt) in plant organism, which strategy is the best for cloning it?
Posted 23 June 2004 - 06:13 AM
synthesize 2 primers in complementry,and anneal to each other, then clone. if you like, you can add restriction sites to the primer so that it's easier to put in a vector.