I'm extracting MIGR1 plasmids using very standard techniques followed by Maxi Pred.
I end up with a nice big pellet of plasmid, at the very least 1ug/uL on nano drop (around 400-500uL volume)
Then I do phenol:chloroform:isoamyl based extraction and still get a large pellet at the end.
I'm having real trouble redissolving the pellet though. I'm trying to redissolve them in elution buffer from the Maxi kit and have left them overnight in the fridge with 500uL of the buffer, then tried shaking them at 20C in the morning, but I can still see the pellets and the nano drop suggests most of it has not dissolved (50ng/ul DNA in the soluble fraction now).
Can anyone suggest where the problem lies and what I can do to rescue the plasmids?
Thanks in advance.