I have a (rather general) question concerning the wet transfer conditions of a western blot. Transfer conditions obviously vary widely depending on many things like transfer buffer formulation, acrylamide concentration and thickness of the gel, size and pI of protein of interest, membrane material etc. and that they ultimately have to be optimized for every single application.
But, when looking through general guidelines of manufacturers I have come across a rather puzzling discrepancy. All details aside, for general wet transfer of "regular" Tris-Glycine gels Life Technologies recommends a constant voltage setting of 25 V for 1-2 h, whereas Bio-Rad recommends constant 30 V for 16 h (or a "high-intensity" setting of 100 V for 1 h).
25 V for 1-2h vs. 30 V for 16h (or 100 V for 1 h)
How is this obvious discrepancy explained? I am confused