I have been trying to find the best technique to get BRCA1 and BRCA2 foci in immunofluorescence staining of cells but with no avail.
I am using the following antibodies:
Calbiochem mouse BRCA-2 (Ab-1) #OP95
Calbiochem mouse BRCA-1 (Ab-1) #OP92
Santa Cruz mouse BRCA-1 (D-9)
Having perused a multitude of literature and attempting different techniques – pre-permeabilisation and different fixing– PFA and MeOH/Ac, different concentrations etc
I have yet to recreate the wonderful images seen in all the other journals.
Does anyone have any advice or tips on which technique is best? Perhaps I should consider using a different antibody?
Thanks in advance for your help!