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Problems with BRCA1 and BRCA2 staining for Immunofluorescence

BRCA1 BRCA2 Immunofluorescence

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#1 GTse

GTse

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Posted 20 May 2014 - 07:12 AM

Hello,

 

I have been trying to find the best technique to get BRCA1 and BRCA2 foci in immunofluorescence staining of cells but with no avail.

 

I am using the following antibodies:

 

Calbiochem mouse BRCA-2 (Ab-1) #OP95

Calbiochem mouse BRCA-1 (Ab-1) #OP92

Santa Cruz mouse BRCA-1 (D-9)

 

Having perused a multitude of literature and attempting different techniques – pre-permeabilisation and different fixing– PFA and MeOH/Ac, different concentrations etc

I have yet to recreate the wonderful images seen in all the other journals.

 

Does anyone have any advice or tips on which technique is best? Perhaps I should consider using a different antibody?

 

Thanks in advance for your help!







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