Dear experts in immunology,
Recently our lab generated a gene knockout mouse. The KO animals are fertile and look completely normal except that they have heavier spleens and lower % eosinophils, which, we hope, are phenotypes. However, eosinophil #/uL in KO animal blood is not significantly different from that in wild type control mouse blood.
In case that a gene KO causes the spleen heavier or reduce % eosinophils, what test/experiments can I try? Since I and my lab have very limited knowledge on immunology, I need some help from immunology experts.
Thanks in advance.