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Mice LTP is not working

EPSP decline

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#1 sudarshanspatil

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Posted 19 May 2014 - 11:30 AM

Dear friend

I am Sudarshan Patil, started to work on mice LTP. Trying to measure fEPSP in vivo. I am getting very good/typical signal from the mice dentate gyrus (Please see attached photo). I tried many different stimulation protocols such as 6x6x6 TBS at 400Hz. or 200Hz x 4 at 10 second interval or 400Hz x3 at 10 second interval. I found increase in population skype but unfortunately decrease in fEPSP.

Any one can suggest me where I am going wrong.

I am loweing electrodes with slow speed. stabilizing signal for 30 mins. baseline recording start at minimal population spike amplitude.

 

Thanks

Sudarshan Patil

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#2 Inmost sun

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Posted 09 August 2014 - 10:45 AM

lower Hz and increase breaks (minutes!)






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