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FITC conjugated phalloidin staining of cells on microcarrier beads


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#1 vidhya

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Posted 18 May 2014 - 09:25 PM

Hi,

 

          I m working on microgravity based cell culture on microcarrier beads. I m trying to visualise F actin in huvecs cultured on dextran based cytodex microcarrier beads. I tried staining the beads with FITC conjugated phalloidin. I m getting very high background from the beads. and hence I m not able visualise the cells on the beads. HAs anyone encountered such problems? Any help would be appreciated

 

 

 

Thanks :)

 

Vidhya



#2 bob1

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Posted 19 May 2014 - 01:21 PM

Some details of what you have already tried would be useful.






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