I was just wondering if someone could help me understand the change in freezing temperature that has seemingly occurred in a 50mM Tris-HCl, pH 8.0 - as compared to distilled water. I went to get a Nickle Column that I had stored in the fridge (it's 4C - confirmed), and liquid over the top of the Nickle resin had frozen.
What's the basic science behind the lowering of freezing temp by Organic Buffers? Theorizing: Tris somehow bound strongly enough (more strongly than H-bonds alone) to some water molecules, allowing the molecules to stay still at a higher temperature than what is observed by water alone...
Is this what is happening? Any additives to prevent this freezing that are compatible with Nickle resin (I don't know if glycerol is compatible?)
I enjoy this trips back to basic science!
Thanks for any help.