Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo

Brevibacillus borstelensis as contaminant in our lab?

contamination

  • Please log in to reply
3 replies to this topic

#1 tretol

tretol

    member

  • Active Members
  • Pip
  • 29 posts
0
Neutral

Posted 18 May 2014 - 03:16 AM

Hello everybody,

 

I isolated bacteria from the environment, did colony PCR and sequencing of the 16S rRNA geneand was surprised that the result was Brevibacillus borstelensis. This is a thermophilic polyethylene degrading bacterium. I again amplified the gene and sequenced, the results were the same. Has anybody ever encountered this species as contamination or has worked with this species and give me some hints? At our lab, we are working with crustaceans, birds and pigs.

 

 

Thank you very much!


Edited by tretol, 18 May 2014 - 03:17 AM.


#2 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 5,664 posts
394
Excellent

Posted 18 May 2014 - 01:08 PM

As it is a thermophile it could come from composting pig faeces.



#3 tretol

tretol

    member

  • Active Members
  • Pip
  • 29 posts
0
Neutral

Posted 21 May 2014 - 10:33 PM

mhh, we have pig brains only. Seems to be a special contaminant



#4 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 5,664 posts
394
Excellent

Posted 22 May 2014 - 01:21 AM

It could be that the people who are getting the pigs brains are bringing it back to the lab?







Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.