I tried to prepare the following medium. It is a modified LB agar. I would like to test if my bacteria are facultatively anaerobic. Hence, I have to add sugars since LB does not contain them. Since I am not sure which sugars my bacteria might metabolize I read some papers and decided for a sugar mix.
I will give the recipe of my media. Please tell me, if any information is incorrect or incomplete. For example: How can I dissolve the soluble starch I used and what is dipotassium phosphate and magnesium sulfate good for (magnesium sulfate maybe as cofactor for enzymes and the formation of cystein and methionin)?
Thank you very much!