1 reply to this topic

[Mariam-Lilia]

Hello,

 

My name is Mariam and I am a PhD student in the first year of my studies.

I have been facing several problems performing the MTT but now I would like to ask you. After the incubation time with the MTT solution, is the colour change visible by the human eye? Or should I put it under the microscope to check if any crystals have been formed?

Because usually I see a yellowish colour even after the 2h incubation.

 

Thank you for your help in advance.

 

Mariam.

[vinclam]

After incubate with MTT solution, there should be no colour changes which can be detected by human eyes.

 

For my protocol, I'll aspirate the MTT solution after incubate for 4 hours and add in DMSO to dissolve the crystal formed. Dark purple colour can easily detected by human eyes immediately after i add in DMSO into the wells. The colour changes will be detected using microplate reader to read the absorbance. Anyhow, i didn't check the colour of MTT solution during its incubation period as it is light-protected during the incubation period.