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MTT and formazan crystals:Can I see the colour change only under the microscope?

MTT. formazan crystals colour change microscope cytotoxicity

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#1 Mariam-Lilia



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Posted 16 May 2014 - 03:02 AM



My name is Mariam and I am a PhD student in the first year of my studies.

I have been facing several problems performing the MTT but now I would like to ask you. After the incubation time with the MTT solution, is the colour change visible by the human eye? Or should I put it under the microscope to check if any crystals have been formed?

Because usually I see a yellowish colour even after the 2h incubation.


Thank you for your help in advance.



#2 vinclam



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Posted 20 August 2014 - 05:03 AM

After incubate with MTT solution, there should be no colour changes which can be detected by human eyes.


For my protocol, I'll aspirate the MTT solution after incubate for 4 hours and add in DMSO to dissolve the crystal formed. Dark purple colour can easily detected by human eyes immediately after i add in DMSO into the wells. The colour changes will be detected using microplate reader to read the absorbance. Anyhow, i didn't check the colour of MTT solution during its incubation period as it is light-protected during the incubation period.

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