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MTT and formazan crystals:Can I see the colour change only under the microscope?

MTT. formazan crystals colour change microscope cytotoxicity

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#1 Mariam-Lilia

Mariam-Lilia

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Posted 16 May 2014 - 03:02 AM

Hello,

 

My name is Mariam and I am a PhD student in the first year of my studies.

I have been facing several problems performing the MTT but now I would like to ask you. After the incubation time with the MTT solution, is the colour change visible by the human eye? Or should I put it under the microscope to check if any crystals have been formed?

Because usually I see a yellowish colour even after the 2h incubation.

 

Thank you for your help in advance.

 

Mariam.







Also tagged with one or more of these keywords: MTT. formazan, crystals, colour change, microscope, cytotoxicity

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