I was expecting 4 clear bands from my enzyme digest but saw almost twice as many bands from what is expected. Can anyone explain what might have caused this and how can I improve that?
Just a side note, I also ran the undigested plasmid in the same gel and there was a single band at the right size.
My enzymatic reaction was: 2h at 37°C (this was suggested by the person I got the plasmid from) with 2 units of BamH1 in a 10ul reaction with 1ug plasmid. The final mix contains 1x BSA and 1x buffer.