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Elisa reading for 24 hrs at 37C

Elisa

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#1 Dima

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Posted 14 May 2014 - 10:38 AM

Hello! Has anyone encountered that a 96 wells plate incubated at 37C for 24hrs in automated elisa reader ended up with wells having volume almost half their initial volume? Is it evaporating? Each well has 300 microl..after 24 hrs it s much less.These Plates have no covers..

In that case i suppose the readings are not accurate??

#2 mdfenko

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Posted 14 May 2014 - 10:55 AM

sounds like evaporation to me.

 

if it's equal for each well then you may be able to make valid comparisons but it's not likely to be equal.


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genius does what it must
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#3 Dima

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Posted 20 May 2014 - 06:04 AM

Thx ! No they are not equal.am repeating the whole thing with plates with lids. I suppose that was not smart to use plates with no lids :(

#4 mdfenko

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Posted 20 May 2014 - 11:16 AM

better to use seals (like these from sigma)


talent does what it can
genius does what it must
i do what i get paid to do





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