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Can you store DNA in loading dye buffer short term?

dna gel electrophoresis dna gel purification buffers storage

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#1 essiyl



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Posted 13 May 2014 - 11:31 AM

I'm running gels for a DNA gel purification but had too much sample to fit in all the available wells.  Unfortunately, I had already mixed my DNA samples with 6x loading dye buffer (w/xylene cyanol and bromophenol blue) and was wondering if it is possible to store the samples short term (for a couple of hours or overnight) until I can run them on a gel.  I have them right now at 4C, but if there is a better temperature to store them please let me know.  The samples contain DNA digests w/approximately 100ug DNA as well as enzyme, buffer, and H2O.  Thanks.

#2 bob1


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Posted 13 May 2014 - 12:48 PM

It should be fine - the solutions are usually buffered to a pH that will maintain DNA more or less indefinitely if stored at -20, and for some weeks at 4.

#3 hobglobin


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Posted 15 May 2014 - 08:25 AM

Depends also a bit on the enzyme, so if it is an enzyme with star activity you should inactivate it or store the samples at -20 to avoid further and unspecific digestions.

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