My lab just ordered the Epigentek kit for global DNA methylation. We are using the standard curve reagents provided with the kit and have gotten a linear regression for our standards only twice out of many tries. We have figured out how to make our microplate reader shake the plate and have waited the required time(s) listed in the protocol before reading the plate, putting in different solutions...etc. I find that there is a big difference in the variability between duplicates and am not sure if a blank is needed or not? The protocol doesn't specify that one is needed but we have included one anyway. Does anyone have any tips on how to get this to work that newbees may not know?