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Using a mouse antibody for IP - should I use Dynabeads with Protein G or with sh

immunoprecipitation Dynabead Protein G Dynabead sheep antimouse Mouse IgG1 κ IP

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#1 Epigeneticist

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Posted 11 May 2014 - 09:05 AM

Hi, 

 

I plan to incorporate BrdU into the DNA of my proliferating human cells in culture, isolate the DNA and IP with an anti-BrdU antibody to capture those DNA sequences.  I am wondering the disadvantages/advantages of using Dynabeads with either Protein G or sheep anti-mouse to capture the mouse anti-BrdU:DNA(BrdU) compexes? Specificity? Sensitivity? Efficiency?  I feel that the two would be almost equal but I am not sure, which is why I am posting this question. 

 

Thanks!



#2 bob1

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Posted 11 May 2014 - 12:45 PM

Most IPs are done with protein G - it gives you less IgG in the final solution and the interaction is with the "other" end of the antibody, so it doesn't interfere with the IP at all.







Also tagged with one or more of these keywords: immunoprecipitation, Dynabead Protein G, Dynabead sheep antimouse, Mouse IgG1 κ, IP

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