Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

Using a mouse antibody for IP - should I use Dynabeads with Protein G or with sh

immunoprecipitation Dynabead Protein G Dynabead sheep antimouse Mouse IgG1 κ IP

  • Please log in to reply
1 reply to this topic

#1 5280



  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 145 posts

Posted 11 May 2014 - 09:05 AM



I plan to incorporate BrdU into the DNA of my proliferating human cells in culture, isolate the DNA and IP with an anti-BrdU antibody to capture those DNA sequences.  I am wondering the disadvantages/advantages of using Dynabeads with either Protein G or sheep anti-mouse to capture the mouse anti-BrdU:DNA(BrdU) compexes? Specificity? Sensitivity? Efficiency?  I feel that the two would be almost equal but I am not sure, which is why I am posting this question. 



#2 bob1


    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 6,670 posts

Posted 11 May 2014 - 12:45 PM

Most IPs are done with protein G - it gives you less IgG in the final solution and the interaction is with the "other" end of the antibody, so it doesn't interfere with the IP at all.

Also tagged with one or more of these keywords: immunoprecipitation, Dynabead Protein G, Dynabead sheep antimouse, Mouse IgG1 κ, IP

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.