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Using a mouse antibody for IP - should I use Dynabeads with Protein G or with sh

immunoprecipitation Dynabead Protein G Dynabead sheep antimouse Mouse IgG1 κ IP

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#1 5280



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Posted 11 May 2014 - 09:05 AM



I plan to incorporate BrdU into the DNA of my proliferating human cells in culture, isolate the DNA and IP with an anti-BrdU antibody to capture those DNA sequences.  I am wondering the disadvantages/advantages of using Dynabeads with either Protein G or sheep anti-mouse to capture the mouse anti-BrdU:DNA(BrdU) compexes? Specificity? Sensitivity? Efficiency?  I feel that the two would be almost equal but I am not sure, which is why I am posting this question. 



#2 bob1


    Thelymitra pulchella

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Posted 11 May 2014 - 12:45 PM

Most IPs are done with protein G - it gives you less IgG in the final solution and the interaction is with the "other" end of the antibody, so it doesn't interfere with the IP at all.

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