Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

3'DNA biotinylation kit


  • Please log in to reply
No replies to this topic

#1 shaunyb

shaunyb

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 21 June 2004 - 06:53 AM

Hey all,

Have been biotinylating DNA using Pierce's 3'DNA end labelling kit. However, I can't detect anything by ECL, not even control samples when i am doing a blot to estiamte labelling efficiency.


Am immobilising DNA to HYbond N membrane

Using 3% BSA in PBS to block membrane, rinsing in PBS Tween and probing with HRP-STreptavidin conjugate (1:500), but not detecting anything by ECl.

Anyone having any similar problems? Anyone have any advice?

Much appreciated

Shaunyb




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.