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3'DNA biotinylation kit

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#1 shaunyb



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Posted 21 June 2004 - 06:53 AM

Hey all,

Have been biotinylating DNA using Pierce's 3'DNA end labelling kit. However, I can't detect anything by ECL, not even control samples when i am doing a blot to estiamte labelling efficiency.

Am immobilising DNA to HYbond N membrane

Using 3% BSA in PBS to block membrane, rinsing in PBS Tween and probing with HRP-STreptavidin conjugate (1:500), but not detecting anything by ECl.

Anyone having any similar problems? Anyone have any advice?

Much appreciated


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