Have been biotinylating DNA using Pierce's 3'DNA end labelling kit. However, I can't detect anything by ECL, not even control samples when i am doing a blot to estiamte labelling efficiency.
Am immobilising DNA to HYbond N membrane
Using 3% BSA in PBS to block membrane, rinsing in PBS Tween and probing with HRP-STreptavidin conjugate (1:500), but not detecting anything by ECl.
Anyone having any similar problems? Anyone have any advice?
3'DNA biotinylation kit
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