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PCR troubleshooting: wrong amplicon size when spiked into sample


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#1 theschu2



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Posted 09 May 2014 - 08:51 AM

Hoping I can get some help here. My graduate student is using real-time PCR with an amplicon of 200bp. Her positive control is the correct length and melting temp but when she spikes already extracted DNA into extracted matrix her amplicon is only 100bp. Any ideas of why this might be occurring?

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