I know it sounds trivial but i have some problems with calculations for the experiment that I've performed today in the lab. I was supposed to do the bioassay for the lysozyme, using 3 samples: crude, precipitation, dialysis and of course standards.
Preparation of satndards: 1mg lysozyme/ml of stock with/ spec activity of 47080 U/mg that needed to be diluted 20 times (I've used a total volume of 500ul, so 25ul in 475 ul of potassiumphosphate buffer)
150 ul of potassiumphosphate buffer
150ul of diluted lysozyme stock
then what is enzyme activity in the standard solution (U/ml)?
I keep making some mistakes and i'm pretty sure of it