I had a beginner's question, new to all of this. While looking at plasmid constructs, I notice sometimes, when 2 proteins need to be co-expressed, they are cloned in as:
Protein A - IRES - Protein B
but other times as:
Protein A - IRES - Protein B - PolyA
What is the purpose of the poly A sequence? Does it boost up protein levels by stabilizing the mRNA?
Thanks a lot!