Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

Which selection agents cause the most and least amount of DNA strand breakage?

Transient transfection Transgene insertion Drug selection Tissue Culture

  • Please log in to reply
No replies to this topic

#1 5280



  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 145 posts

Posted 06 May 2014 - 07:29 PM

I want to perform a transient transfection in a human cell line and then a short drug selection (i.e. puro, G418 etc few days) to enrich for transfected cells. However, I want to prevent the plasmid from integrating. Which drug (i.e. G418, Puro, zeomycin, hygro, etc) causes the most and which ones cause the least amount of DNA breaks? I assume the drugs that cause the least will also result in less plasmid integration. Any information on this?



Edited by Epigeneticist, 07 May 2014 - 05:12 AM.

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.