I'm working on my bachelor thesis and finally got some good clones to continue with. I wanted to store them in glycerol stocks, but I guess I missunderstood my adviser. I took 1400µl glycerol and 600µl overnight culture (so it's 70:30). But from others I know that they take more culture than glycerol... so I wonder if this much glycerol is very bad for my cultures? It's 80% glycerol.
Please excuse my english!