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Transfection Enhancer Efficiency


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#1 TrafEn

TrafEn

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Posted 30 April 2014 - 04:01 AM

Hi All, 

There are a couple of transfection enhancers available for lipid-based transfection, nucleobooster (BCC), Effectene (Qiagen) and Lipofectamine Plus (Life Technologies). Does anyone have experience working with them? And what is the transfection efficiency and toxicity with these enhancers?

 

 

Edit - You posted this same question in three different subforums. There is no need for that. New questions are displayed on the homepage and will be viewed/answered regardless of the subforum that are in. -JerryShelly1



#2 Olivia Zabel

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Posted 19 November 2014 - 12:12 AM

Hi,

 

for siRNA/miRNA as well as for plasmid/shRNA/mRNA transfection I use a new kind of transfection reagent. Its called: Viromer and was developed from Lipocalyx (www.lipocalyx.de).

Viromers are synthetic polymers zero in charge and very gently to cells. Due to an active endosome escape mechanism I get higher transfection efficiency compared to standard transfection reagents.

 

Please find attached some data and information.

Attached Files


Edited by Olivia Zabel, 19 November 2014 - 12:54 AM.





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