The attached file is the melting curve of my qPCR. The purple curves belong to housekeeping gene. The other colors are my samples, most of them peak at 75C, same temp known for primer-dimer.
Now, my housekeeping gene Ct value is at 15-16 cycle (not shown here), but the samples rise at 26-28 cycle. I thought primer-dimer usually rises after 30 cycles. So what are these peaks? Could this be because of low concentration of template?