I want to do an Ip of my protein. ussually when I do from a lysate I use 500ug of my total protein.
In this case I have the pure purifiy protein. So how much of protein houd I use? is 10ug good.
aslo for the IP usually people mixe the Primary antibody with the protein for 2 H or O/N, then add the protein Aagarose bead for 2 hours.
is it possible to fo first antibody+ bead O/N then next day the protein.